Molecule detected in formalin fixed tissue by antibodies MT1, DF-T1, and L60 (Leu-22) corresponds to CD43 antigen.

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Molecule detected in formalin fixed tissue by antibodies MT1, DF-T1, and L60 (Leu-22) corresponds to CD43 antigen.

Three monoclonal antibodies MT1, L60 (Leu-22), and DF-T1, were reported independently as recognising human T cells in routinely processed, paraffin wax embedded tissue. The present study was performed to compare these three reagents in terms of their immunocytochemical reactions and target molecule(s). On Western blotting of white cell extracts the three antibodies reacted with antigens of the ...

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Generation of monoclonal antibodies recognizing neuronal elements in formalin-fixed paraffin-embedded human tissue.

We used formalin-fixed human spinal cord and dorsal root ganglia as immunogens to generate monoclonal antibodies (mAb) which immunohistochemically react with neurons in formalin-fixed human tissue sections. Three of the mAb recognized all neuronal populations studied, including those in spinal cord, dorsal root ganglia, cerebellum, and cerebrum. A fourth mAb recognized neurons within spinal cor...

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Analysis of formalin-fixed, paraffin-embedded (FFPE) tissue via proteomic techniques and misconceptions of antigen retrieval.

Since emerging in the late 19(th) century, formaldehyde fixation has become a standard method for preservation of tissues from clinical samples. The advantage of formaldehyde fixation is that fixed tissues can be stored at room temperature for decades without concern for degradation. This has led to the generation of huge tissue banks containing thousands of clinically significant samples. Here...

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Bacteria detected on surfaces of formalin fixed anatomy cadavers.

The purpose of this study is to determine if anatomy cadavers fixed in a formalin solution are a possible source of introduction of microorganisms into the anatomy laboratory. Routinely preserved cadavers were sampled for microbiological contaminates prior to examination and dissection by anatomy students. Regions sampled include the axilla, oral/nasal cavity, and inguinal/perineal region. Usin...

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Establishment of monoclonal anti-human CD26 antibodies suitable for immunostaining of formalin-fixed tissue

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1989

ISSN: 0021-9746

DOI: 10.1136/jcp.42.9.953